• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Gas-phase fragmentation of the N-oxide and N-hydroxylated derivatives of retrorsine using liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry
【2h】

Gas-phase fragmentation of the N-oxide and N-hydroxylated derivatives of retrorsine using liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry

机译：液相色谱/电喷雾电离四极杆飞行时间串联质谱分析逆转录酶的N-氧化物和N-羟基化衍生物的气相裂解

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report the electrospray ionization mass spectrometry and low-energy collision-induced dissociation tandem mass spectrometry (CID-MS/MS) analysis of a pyrrolizidine alkaloid extract containing both retrorsine [C18H25NO6] and its N-oxide [C18H25NO7] and N-hydroxyl [C18H26NO7] derivatives measured with a QqTOFMS hybrid instrument. Methods A solution of the pyrrolizidine alkaloid extract containing retrorsine and its N-oxide and N-hydroxyl derivatives was directly infused into an electrospray ionization-quadrupole-time-of-flight (ESI-QTOF) mass spectrometer and product ion scans of the protonated molecules of each species were acquired. Labile protons of each compound were deuterated and computational energy calculations of the proposed structures of the product ions were used to determine the fragmentation pathways of retrorsine and its N-oxide and N-hydroxyl derivatives. Results ESI-MS of the pyrrolizidine alkaloid extract containing retrorsine and its N-oxide and N-hydroxyl derivatives afforded the protonated retrorsine [M1+H]+ at m/z 352.1760 and the protonated retrorsine N-oxide [M2+H]+ at m/z 368.1631 in addition to the formation of the unexpected protonated N-hydroxyl radical [M3+H]+• at m/z 369.1686. CID-MS/MS of this series of protonated molecules allowed the evaluation of their gas-phase fragmentations and the establishment of their fragmentation pathways. It was also found that several product ions could be assigned to different structures. Deuterium exchange and computational energy calculations allowed us to determine the most probable structures for the characterized product ions. Conclusions To our knowledge, the identification of the protonated retrorsine N-hydroxyl radical [M3+H]+• is reported for the first time. In addition, the MS/MS results can be used for the identification of retrorsine and its N-oxide and N-hydroxyl derivatives in different complex biological matrices
机译：我们报告了吡咯烷定生物碱提取物的电喷雾电离质谱和低能碰撞诱导解离串联质谱分析（CID-MS / MS），其中既包含逆转录酶[C18H25NO6]，又包含其N-氧化物[C18H25NO7]和N-羟基[使用QqTOFMS混合仪器测量的C18H26NO7]衍生物。方法将含有逆转录酶及其N-氧化物和N-羟基衍生物的吡咯嗪核生物碱提取物溶液直接注入电喷雾电离四极杆飞行时间（ESI-QTOF）质谱仪中，并对质子化分子进行产物离子扫描每个物种的。将每种化合物的不稳定质子氘化，并使用拟议的产物离子结构的计算能量计算来确定逆转录酶及其N-氧化物和N-羟基衍生物的裂解途径。结果含有逆转录酶及其N-氧化物和N-羟基衍生物的吡咯烷核生物碱提取物的ESI-MS在m / z 352.1760处得到质子化的逆转录酶[M1 + H] +和在14/20时得到质子化的逆转录酶N-氧化物[M2 + H] + m / z 368.1631以及m / z 369.1686处意外的质子化N-羟基自由基[M3 + H] +•的形成。该系列质子化分子的CID-MS / MS可以评估其气相碎片化并建立其碎片化途径。还发现几种产物离子可以分配给不同的结构。氘交换和计算能的计算使我们能够确定特征产物离子的最可能结构。结论据我们所知，首次报道了质子化逆转录酶N-羟基自由基[M3 + H] +•的鉴定。此外，MS / MS结果可用于鉴定不同复杂生物基质中的逆转录酶及其N-氧化物和N-羟基衍生物

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号